Pipette Calibration: What You Need To Know - BioTechniques
WEBJun 21, 2024 · ISO8655:2022: the importance of pipette calibration. In this Expert Interview, we learn about how pipette calibration impacts the user and the importance of regular pipette maintenance and testing in the lab. Joni Åke, Product Manager for electronic pipettes at Sartorius, shares his expertise, discussing how …
More compact CRISPR enzyme engineered - biotechniques.com
WEBOct 4, 2023 · A collaboration led by researchers at the University of Tokyo (Japan) has engineered a compact CRISPR enzyme using deep mutational scanning, a method for the multiplex measurement of thousands of protein variants, and structural analysis methods. The resulting enzyme, enAsCas12f, is a third of the size of Cas9 and just as effective.
Aims and scope - BioTechniques
WEBBioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science ...
A Guide to Flow Cytometry - BioTechniques
WEBDec 12, 2022 · Flow cytometry (Flow = fluidics, cyto = cell and metry = measure) is a revolutionary technique that was forged at the forefront of the biotechnology explosion in the 1960s. It has since established robust frontiers in the practice, policy and cutting-edge innovation of biology and medicine. The game-changing leaps in cytometry have enabled ...
Solving Environmental Problems with New Technology
WEBMay 7, 2020 · This new technology, known as cold-SNAP, improves evaporative cooling systems through the use of 3D-printed ceramic coated on one side in a hydrophobic nanoscale material. This results in an effective heat exchange unit that is able to isolate evaporating water from cooled air. The coating acts as a water vapor barrier, limiting humidification ...
CRISPR-Combo Both Edits and Activates Genes - BioTechniques
WEBJun 29, 2022 · CRISPR-Combo combines gene activation and editing. Simultaneous gene activation and gene editing for different targets in plant genomes is made possible with CRISPR-Combo. Since the discovery of CRISPR/Cas9 a decade ago, scientists have been able to change the genetic code of living organisms; however, this first generation of technology could ...
How Does Stolonization Work? - BioTechniques
WEBNov 30, 2023 · Stolonization describes the process by which an organism develops gonads in the posterior segment of its body, before detaching that segment from the body. Complete with a head, eyes and swimming bristles, this structure is referred to as a stolon. Having detached, the syllid worm’s stolon swims off …
How Early Life Thrived Without Oxygen - BioTechniques
WEBJan 11, 2017 · According to Planavsky, oxygen saturated the surface of oceans and the atmosphere, but these sulfur-oxidizing bacteria thrived best at the interface between oxic and oxygen-poor waters. The evolution of the environment changed the course of evolution for most life on Earth. “A lot of people don’t think about how the Earth has changed so ...
What is Western Blotting? The Story of the Western Blot
WEBJul 18, 2018 · The technique involves the identification and characterization of DNA-binding proteins using DNA probes (Southern) and protein blotting (western) and was first described in 1980. This method was published before the western blot. Yet, it still alludes to it in its name, reaffirming the popularity of the western blot.
The Nanoimager: single-molecule imaging at your fingertips
WEBApr 22, 2020 · The Nanoimager from Oxford NanoImaging (ONI) is a desktop-compatible, super-resolution, single-molecule fluorescence microscope capable of resolving structures with 20 nm resolution and a localization precision of 2 nm. It supports various modes of operation: single-molecule localization-based super-resolution for quantitative cellular imaging; single-particle tracking in live cells; DMD-based ...